T7
T7rnap-G

Part:BBa_I716104

Designed by: David Tulga   Group: iGEM07_Berkeley_UC   (2007-06-09)

T7rnap (T7 RNA Polymerase) - GTG Start Codon

This is the T7 RNA Polymerase gene (GTG Start), and will transcribe any genes with a T7 Promoter. It provides very good PoPS (Polymerases Per Second) output, and it is highly orthogonal to E. Coli's natural transcription system. This variation has a GTG start codon, and so will not produce as much output as the ATG start codon variation. It is also less toxic, but still potentially unstable in a -80 stock.

For further information, [http://2007.igem.org/BerkiGEM2007Present3 see here].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None